Aggrescan4D: d3cfe2efc86c51d

Project name: d3cfe2efc86c51d

Status: done

Started: 2026-05-20 07:37:39

Chain sequence(s)	A: EVQLVESGGGLVKPGGSLKLSCAASGYTFTSYVMHWVRQAPGKGLEWIGYINPYNDGTKYNEKFQGRVTISSDKSISTAYMELSSLRSEDTAMYYCARGTYYYGTRVFDYWGQGTLVTVSSGGGGSGGGGSGGGGSDIVMTQSPATLSLSPGERATLSCRSSKSLQNVNGNTYLYWFQQKPGQSPQLLIYRMSNLNSGVPDRFSGSGSGTEFTLTISSLEPEDFAVYYCMQHLEYPITFGAGTKLEIKSGGGGSEVQLVESGGALVKPGGSLRLSCAASGFTFTEAWMHWVRQAPGKQLEWVAQIKDRSQTYATYYAESVKGRFTISRDDSKNTLYLQMNSLKTEDTAVYYCRGVYYANAPFDYWGQGTLVTVSSGGGGSGGGGSGGGGSDIVMTQSPLSLPVTPGEPASISCRSSQSLVHNTGNTYLSWYLQKPGQSPQSLIYKVSNRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCGQGTQAPFTFGSGTKVEIKGGGGDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLYITREPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALAAPIEKTISKAKGQPREPQVYTLPPCREEMTKNQVSLWCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK B: EVQLLESGGGLVQPGGSLRLSCAASGFTFDDYAIGWFRQAPGKEREGVSCIRVSDGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAAGSLYTCVQSIVWPARPYYDMDYWGQGTLVTVSSEPKSSDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLYITREPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALAAPIEKTISKAKGQPREPQVCTLPPSREEMTKNQVSLSCAVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLVSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK input PDB
Selected Chain(s)	A,B
Distance of aggregation	10 Å
FoldX usage	Yes
pH calculations	No
alphaCutter usage	No
Dynamic mode	No
Automated mutations	Yes
Downloads	Download all the data

Simulation log

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[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:01)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:01)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with all chain(s) selected           (00:00:01)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:01)
[INFO]       FoldX:    Starting FoldX energy minimization                                          (00:00:01)
[INFO]       Analysis: Starting Aggrescan4D on folded.pdb                                          (00:14:01)
[INFO]       AutoMut:  Residue number 126 from chain B and a score of 1.721 (leucine) selected for 
                       automated mutation                                                          (00:14:12)
[INFO]       AutoMut:  Residue number 370 from chain A and a score of 1.474 (leucine) selected for 
                       automated mutation                                                          (00:14:12)
[INFO]       AutoMut:  Residue number 265 from chain A and a score of 1.405 (leucine) selected for 
                       automated mutation                                                          (00:14:12)
[INFO]       AutoMut:  Residue number 11 from chain B and a score of 1.359 (leucine) selected for  
                       automated mutation                                                          (00:14:12)
[INFO]       AutoMut:  Residue number 76 from chain A and a score of 1.331 (isoleucine) selected   
                       for automated mutation                                                      (00:14:12)
[INFO]       AutoMut:  Residue number 116 from chain A and a score of 1.315 (leucine) selected for 
                       automated mutation                                                          (00:14:12)
[INFO]       AutoMut:  Mutating residue number 126 from chain B (leucine) into glutamic acid       (00:14:12)
[INFO]       AutoMut:  Mutating residue number 370 from chain A (leucine) into glutamic acid       (00:14:12)
[INFO]       AutoMut:  Mutating residue number 126 from chain B (leucine) into aspartic acid       (00:14:12)
[INFO]       AutoMut:  Mutating residue number 126 from chain B (leucine) into arginine            (00:14:38)
[INFO]       AutoMut:  Mutating residue number 370 from chain A (leucine) into lysine              (00:14:41)
[INFO]       AutoMut:  Mutating residue number 126 from chain B (leucine) into lysine              (00:14:42)
[INFO]       AutoMut:  Mutating residue number 370 from chain A (leucine) into aspartic acid       (00:15:19)
[INFO]       AutoMut:  Mutating residue number 265 from chain A (leucine) into glutamic acid       (00:15:19)
[INFO]       AutoMut:  Mutating residue number 265 from chain A (leucine) into aspartic acid       (00:15:32)
[INFO]       AutoMut:  Mutating residue number 370 from chain A (leucine) into arginine            (00:15:44)
[INFO]       AutoMut:  Mutating residue number 265 from chain A (leucine) into lysine              (00:15:45)
[INFO]       AutoMut:  Mutating residue number 265 from chain A (leucine) into arginine            (00:15:56)
[INFO]       AutoMut:  Mutating residue number 11 from chain B (leucine) into glutamic acid        (00:16:15)
[INFO]       AutoMut:  Mutating residue number 11 from chain B (leucine) into aspartic acid        (00:16:20)
[INFO]       AutoMut:  Mutating residue number 76 from chain A (isoleucine) into glutamic acid     (00:16:25)
[INFO]       AutoMut:  Mutating residue number 11 from chain B (leucine) into lysine               (00:16:47)
[INFO]       AutoMut:  Mutating residue number 11 from chain B (leucine) into arginine             (00:16:50)
[INFO]       AutoMut:  Mutating residue number 76 from chain A (isoleucine) into lysine            (00:16:55)
[INFO]       AutoMut:  Mutating residue number 76 from chain A (isoleucine) into aspartic acid     (00:17:25)
[INFO]       AutoMut:  Mutating residue number 116 from chain A (leucine) into glutamic acid       (00:17:25)
[INFO]       AutoMut:  Mutating residue number 116 from chain A (leucine) into aspartic acid       (00:17:35)
[INFO]       AutoMut:  Mutating residue number 76 from chain A (isoleucine) into arginine          (00:17:51)
[INFO]       AutoMut:  Mutating residue number 116 from chain A (leucine) into lysine              (00:17:52)
[INFO]       AutoMut:  Mutating residue number 116 from chain A (leucine) into arginine            (00:18:01)
[INFO]       AutoMut:  Effect of mutation residue number 126 from chain B (leucine) into glutamic  
                       acid: Energy difference: 0.2939 kcal/mol, Difference in average score from  
                       the base case: -0.0123                                                      (00:18:39)
[INFO]       AutoMut:  Effect of mutation residue number 126 from chain B (leucine) into lysine:   
                       Energy difference: -0.2293 kcal/mol, Difference in average score from the   
                       base case: -0.0112                                                          (00:18:39)
[INFO]       AutoMut:  Effect of mutation residue number 126 from chain B (leucine) into aspartic  
                       acid: Energy difference: 0.8226 kcal/mol, Difference in average score from  
                       the base case: -0.0113                                                      (00:18:39)
[INFO]       AutoMut:  Effect of mutation residue number 126 from chain B (leucine) into arginine: 
                       Energy difference: -0.8452 kcal/mol, Difference in average score from the   
                       base case: -0.0120                                                          (00:18:39)
[INFO]       AutoMut:  Effect of mutation residue number 370 from chain A (leucine) into glutamic  
                       acid: Energy difference: 0.3824 kcal/mol, Difference in average score from  
                       the base case: -0.0103                                                      (00:18:39)
[INFO]       AutoMut:  Effect of mutation residue number 370 from chain A (leucine) into lysine:   
                       Energy difference: 0.1616 kcal/mol, Difference in average score from the    
                       base case: -0.0113                                                          (00:18:39)
[INFO]       AutoMut:  Effect of mutation residue number 370 from chain A (leucine) into aspartic  
                       acid: Energy difference: 0.6507 kcal/mol, Difference in average score from  
                       the base case: -0.0098                                                      (00:18:39)
[INFO]       AutoMut:  Effect of mutation residue number 370 from chain A (leucine) into arginine: 
                       Energy difference: 0.0308 kcal/mol, Difference in average score from the    
                       base case: -0.0110                                                          (00:18:39)
[INFO]       AutoMut:  Effect of mutation residue number 265 from chain A (leucine) into glutamic  
                       acid: Energy difference: 0.3497 kcal/mol, Difference in average score from  
                       the base case: -0.0113                                                      (00:18:39)
[INFO]       AutoMut:  Effect of mutation residue number 265 from chain A (leucine) into lysine:   
                       Energy difference: 0.1402 kcal/mol, Difference in average score from the    
                       base case: -0.0106                                                          (00:18:39)
[INFO]       AutoMut:  Effect of mutation residue number 265 from chain A (leucine) into aspartic  
                       acid: Energy difference: 0.6204 kcal/mol, Difference in average score from  
                       the base case: -0.0114                                                      (00:18:39)
[INFO]       AutoMut:  Effect of mutation residue number 265 from chain A (leucine) into arginine: 
                       Energy difference: -0.3310 kcal/mol, Difference in average score from the   
                       base case: -0.0128                                                          (00:18:39)
[INFO]       AutoMut:  Effect of mutation residue number 11 from chain B (leucine) into glutamic   
                       acid: Energy difference: 0.3603 kcal/mol, Difference in average score from  
                       the base case: -0.0113                                                      (00:18:39)
[INFO]       AutoMut:  Effect of mutation residue number 11 from chain B (leucine) into lysine:    
                       Energy difference: 0.4485 kcal/mol, Difference in average score from the    
                       base case: -0.0109                                                          (00:18:39)
[INFO]       AutoMut:  Effect of mutation residue number 11 from chain B (leucine) into aspartic   
                       acid: Energy difference: 0.6987 kcal/mol, Difference in average score from  
                       the base case: -0.0113                                                      (00:18:39)
[INFO]       AutoMut:  Effect of mutation residue number 11 from chain B (leucine) into arginine:  
                       Energy difference: 0.2251 kcal/mol, Difference in average score from the    
                       base case: -0.0114                                                          (00:18:39)
[INFO]       AutoMut:  Effect of mutation residue number 76 from chain A (isoleucine) into         
                       glutamic acid: Energy difference: 0.9431 kcal/mol, Difference in average    
                       score from the base case: -0.0102                                           (00:18:39)
[INFO]       AutoMut:  Effect of mutation residue number 76 from chain A (isoleucine) into lysine: 
                       Energy difference: -0.6153 kcal/mol, Difference in average score from the   
                       base case: -0.0101                                                          (00:18:39)
[INFO]       AutoMut:  Effect of mutation residue number 76 from chain A (isoleucine) into         
                       aspartic acid: Energy difference: 0.3222 kcal/mol, Difference in average    
                       score from the base case: -0.0101                                           (00:18:39)
[INFO]       AutoMut:  Effect of mutation residue number 76 from chain A (isoleucine) into         
                       arginine: Energy difference: -0.6253 kcal/mol, Difference in average score  
                       from the base case: -0.0094                                                 (00:18:39)
[INFO]       AutoMut:  Effect of mutation residue number 116 from chain A (leucine) into glutamic  
                       acid: Energy difference: 0.4981 kcal/mol, Difference in average score from  
                       the base case: -0.0116                                                      (00:18:39)
[INFO]       AutoMut:  Effect of mutation residue number 116 from chain A (leucine) into lysine:   
                       Energy difference: -0.0888 kcal/mol, Difference in average score from the   
                       base case: -0.0109                                                          (00:18:39)
[INFO]       AutoMut:  Effect of mutation residue number 116 from chain A (leucine) into aspartic  
                       acid: Energy difference: 1.0714 kcal/mol, Difference in average score from  
                       the base case: -0.0101                                                      (00:18:39)
[INFO]       AutoMut:  Effect of mutation residue number 116 from chain A (leucine) into arginine: 
                       Energy difference: 0.0278 kcal/mol, Difference in average score from the    
                       base case: -0.0113                                                          (00:18:39)
[INFO]       Main:     Simulation completed successfully.                                          (00:18:49)

Note Your prediction results will be deleted after 360 days.

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Show buried residues

Minimal score value: -3.8739
Maximal score value: 1.7214
Average score: -0.6817
Total score value: -747.1309

Download the table (csv)

The table below lists A4D score for protein residues. Residues with A4D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan4D score

1	E	A	-2.6225
2	V	A	-1.3376
3	Q	A	-1.3858
4	L	A	0.0000
5	V	A	0.6203
6	E	A	0.0000
7	S	A	-0.2618
8	G	A	-0.6169
9	G	A	0.0940
10	G	A	0.8463
11	L	A	1.3008
12	V	A	-0.2910
13	K	A	-1.9059
14	P	A	-1.8711
15	G	A	-1.4912
16	G	A	-1.1483
17	S	A	-1.2491
18	L	A	-1.0768
19	K	A	-2.1079
20	L	A	0.0000
21	S	A	-0.4178
22	C	A	0.0000
23	A	A	0.5020
24	A	A	0.0000
25	S	A	-0.5591
26	G	A	-1.4761
27	Y	A	-0.6918
28	T	A	-0.1648
29	F	A	0.0000
30	T	A	-0.2092
31	S	A	0.0000
32	Y	A	0.5317
33	V	A	0.0000
34	M	A	0.0000
35	H	A	0.0000
36	W	A	0.0000
37	V	A	0.0000
38	R	A	0.0000
39	Q	A	0.0000
40	A	A	-0.9022
41	P	A	-0.9498
42	G	A	-1.4146
43	K	A	-2.0755
44	G	A	-1.0487
45	L	A	0.0000
46	E	A	-0.5422
47	W	A	0.0000
48	I	A	0.0000
49	G	A	0.0000
50	Y	A	0.0000
51	I	A	0.0000
52	N	A	0.0000
53	P	A	0.0000
54	Y	A	-0.3130
55	N	A	-1.5388
56	D	A	-2.0279
57	G	A	-1.3856
58	T	A	-1.1557
59	K	A	-1.4356
60	Y	A	-1.4833
61	N	A	-1.9211
62	E	A	-3.3713
63	K	A	-3.1732
64	F	A	0.0000
65	Q	A	-2.7919
66	G	A	-1.6918
67	R	A	-1.5780
68	V	A	0.0000
69	T	A	-0.9777
70	I	A	0.0000
71	S	A	-0.7569
72	S	A	-0.7799
73	D	A	-0.8859
74	K	A	-0.9093
75	S	A	0.0241
76	I	A	1.3309
77	S	A	0.2434
78	T	A	0.0000
79	A	A	0.0000
80	Y	A	-0.6415
81	M	A	0.0000
82	E	A	-1.5819
83	L	A	0.0000
84	S	A	-1.1364
85	S	A	-1.1867
86	L	A	0.0000
87	R	A	-2.6106
88	S	A	-2.0635
89	E	A	-2.0842
90	D	A	0.0000
91	T	A	-0.3707
92	A	A	0.0000
93	M	A	0.5664
94	Y	A	0.0000
95	Y	A	0.0000
96	C	A	0.0000
97	A	A	0.0000
98	R	A	0.0000
99	G	A	0.0000
100	T	A	0.4359
101	Y	A	1.0406
102	Y	A	0.7129
103	Y	A	0.3717
104	G	A	0.0000
105	T	A	-0.2268
106	R	A	-0.6000
107	V	A	0.0000
108	F	A	0.0000
109	D	A	-0.3148
110	Y	A	-0.1907
111	W	A	0.0000
112	G	A	0.0000
113	Q	A	-1.1550
114	G	A	-0.2554
115	T	A	0.4031
116	L	A	1.3152
117	V	A	0.0000
118	T	A	0.2223
119	V	A	0.0000
120	S	A	-1.0174
121	S	A	-1.5439
122	G	A	-1.2537
123	G	A	-1.1544
124	G	A	-1.1631
125	G	A	-1.2416
126	S	A	-1.0214
127	G	A	-1.6051
128	G	A	-1.1968
129	G	A	-1.3322
130	G	A	-1.1908
131	S	A	-1.0333
132	G	A	-1.4818
133	G	A	-1.6313
134	G	A	-1.3174
135	G	A	-1.6213
136	S	A	-1.1125
137	D	A	-1.2350
138	I	A	0.0000
139	V	A	0.9004
140	M	A	0.0000
141	T	A	-0.3478
142	Q	A	0.0000
143	S	A	-0.4572
144	P	A	-0.2515
145	A	A	-0.1789
146	T	A	-0.4614
147	L	A	-0.1195
148	S	A	-0.2847
149	L	A	-0.2017
150	S	A	-0.8175
151	P	A	-0.9806
152	G	A	-1.1607
153	E	A	0.0000
154	R	A	-0.7995
155	A	A	0.0000
156	T	A	-0.0648
157	L	A	0.0000
158	S	A	-0.8077
159	C	A	0.0000
160	R	A	-2.1650
161	S	A	0.0000
162	S	A	-0.9956
163	K	A	-1.7850
164	S	A	-1.4552
165	L	A	0.0000
166	Q	A	-0.9853
167	N	A	-0.5334
168	V	A	-0.2384
169	N	A	-0.5945
170	G	A	-0.9708
171	N	A	-0.8601
172	T	A	0.0000
173	Y	A	0.0000
174	L	A	0.0000
175	Y	A	0.0000
176	W	A	0.0000
177	F	A	0.0000
178	Q	A	0.0000
179	Q	A	0.0000
180	K	A	-1.2613
181	P	A	-0.8936
182	G	A	-1.3332
183	Q	A	-1.8453
184	S	A	-1.2580
185	P	A	0.0000
186	Q	A	-0.8956
187	L	A	0.0000
188	L	A	0.0000
189	I	A	0.0000
190	Y	A	-0.3592
191	R	A	-0.6970
192	M	A	0.0000
193	S	A	-0.3803
194	N	A	-0.5804
195	L	A	-0.2266
196	N	A	0.0000
197	S	A	-0.4762
198	G	A	-0.6661
199	V	A	-0.4393
200	P	A	-0.7014
201	D	A	-0.9314
202	R	A	-0.8573
203	F	A	0.0000
204	S	A	0.0000
205	G	A	0.0000
206	S	A	0.0000
207	G	A	-1.0091
208	S	A	-1.1983
209	G	A	-1.4054
210	T	A	-1.9609
211	E	A	-2.6696
212	F	A	0.0000
213	T	A	0.0000
214	L	A	0.0000
215	T	A	0.0000
216	I	A	0.0000
217	S	A	0.0000
218	S	A	-0.9758
219	L	A	0.0000
220	E	A	-1.8431
221	P	A	-1.2829
222	E	A	-1.9346
223	D	A	0.0000
224	F	A	-0.5277
225	A	A	0.0000
226	V	A	-0.2345
227	Y	A	0.0000
228	Y	A	0.0000
229	C	A	0.0000
230	M	A	0.0000
231	Q	A	0.0000
232	H	A	0.0000
233	L	A	0.0000
234	E	A	-0.6185
235	Y	A	-0.3260
236	P	A	-0.9207
237	I	A	0.0000
238	T	A	0.1944
239	F	A	0.3942
240	G	A	0.0000
241	A	A	-0.2091
242	G	A	0.0000
243	T	A	0.0000
244	K	A	-0.7098
245	L	A	0.0000
246	E	A	-0.4871
247	I	A	0.7925
248	K	A	-0.8107
249	S	A	-0.7645
250	G	A	-1.0651
251	G	A	-1.3155
252	G	A	-1.5025
253	G	A	-1.7387
254	S	A	-1.6991
255	E	A	-2.4743
256	V	A	0.0000
257	Q	A	-1.3810
258	L	A	0.0000
259	V	A	0.6454
260	E	A	0.0000
261	S	A	-0.2503
262	G	A	-0.5790
263	G	A	0.2226
264	A	A	0.6210
265	L	A	1.4049
266	V	A	-0.1720
267	K	A	-1.7821
268	P	A	-1.7284
269	G	A	-1.4387
270	G	A	-1.0750
271	S	A	-1.3823
272	L	A	-1.1196
273	R	A	-2.1544
274	L	A	0.0000
275	S	A	-0.4612
276	C	A	0.0000
277	A	A	-0.2265
278	A	A	0.0000
279	S	A	-1.0385
280	G	A	-1.2223
281	F	A	0.0000
282	T	A	-0.4312
283	F	A	0.0000
284	T	A	-1.3364
285	E	A	-1.2942
286	A	A	0.0000
287	W	A	-0.3157
288	M	A	0.0000
289	H	A	0.0000
290	W	A	0.0000
291	V	A	0.0000
292	R	A	0.0000
293	Q	A	-0.7681
294	A	A	-1.1227
295	P	A	-0.8926
296	G	A	-1.5213
297	K	A	-2.3911
298	Q	A	-1.6711
299	L	A	0.0000
300	E	A	-1.1622
301	W	A	0.0000
302	V	A	0.0000
303	A	A	0.0000
304	Q	A	0.0000
305	I	A	0.0000
306	K	A	-0.7452
307	D	A	-1.5236
308	R	A	-2.3601
309	S	A	-1.5922
310	Q	A	-1.4719
311	T	A	-1.0099
312	Y	A	-0.5912
313	A	A	-0.2816
314	T	A	0.2124
315	Y	A	0.1450
316	Y	A	-0.6425
317	A	A	0.0000
318	E	A	-2.6307
319	S	A	-2.0246
320	V	A	0.0000
321	K	A	-2.4169
322	G	A	-1.7028
323	R	A	-1.6175
324	F	A	0.0000
325	T	A	-0.7900
326	I	A	0.0000
327	S	A	-0.2547
328	R	A	-0.9500
329	D	A	-1.2735
330	D	A	-1.6470
331	S	A	-1.4110
332	K	A	-2.2489
333	N	A	-1.5996
334	T	A	-0.9251
335	L	A	0.0000
336	Y	A	-0.5599
337	L	A	0.0000
338	Q	A	-1.3006
339	M	A	0.0000
340	N	A	-1.5158
341	S	A	-1.2720
342	L	A	0.0000
343	K	A	-2.2174
344	T	A	-1.9916
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278	Q	B	-1.9932
279	V	B	0.0000
280	S	B	0.0000
281	L	B	0.0000
282	S	B	0.0000
283	C	B	0.0000
284	A	B	0.0000
285	V	B	0.0000
286	K	B	-0.6613
287	G	B	-1.1746
288	F	B	0.0000
289	Y	B	-1.1205
290	P	B	0.0000
291	S	B	-0.2706
292	D	B	-0.8788
293	I	B	-0.4572
294	A	B	0.0000
295	V	B	0.0000
296	E	B	-1.0205
297	W	B	0.0000
298	E	B	-1.1868
299	S	B	0.0000
300	N	B	-1.5333
301	G	B	-1.2945
302	Q	B	-1.2641
303	P	B	-0.8601
304	E	B	0.0000
305	N	B	-0.6622
306	N	B	-0.4111
307	Y	B	-0.3701
308	K	B	-0.6068
309	T	B	-0.3165
310	T	B	0.0000
311	P	B	-0.0421
312	P	B	-0.0597
313	V	B	0.0000
314	L	B	-0.9482
315	D	B	-1.4517
316	S	B	-1.8511
317	D	B	-2.0650
318	G	B	-1.4126
319	S	B	0.0000
320	F	B	-0.2995
321	F	B	0.0000
322	L	B	0.0000
323	V	B	0.0000
324	S	B	0.0000
325	K	B	0.0000
326	L	B	0.0000
327	T	B	-0.4367
328	V	B	0.0000
329	D	B	-2.1346
330	K	B	-2.4291
331	S	B	-2.0876
332	R	B	-1.7693
333	W	B	0.0000
334	Q	B	-2.2903
335	Q	B	-2.0644
336	G	B	-1.1110
337	N	B	-0.7457
338	V	B	0.3115
339	F	B	0.0000
340	S	B	0.0000
341	C	B	0.0000
342	S	B	0.0000
343	V	B	0.0000
344	M	B	0.0000
345	H	B	0.0000
346	E	B	-1.0551
347	A	B	-1.4682
348	L	B	-1.4153
349	H	B	-1.7708
350	N	B	-1.6680
351	H	B	-1.1841
352	Y	B	-0.4420
353	T	B	-0.6862
354	Q	B	-1.0648
355	K	B	-0.9965
356	S	B	-0.4412
357	L	B	0.0000
358	S	B	-0.0744
359	L	B	-0.3085
360	S	B	-0.5960
361	P	B	-1.2282
362	G	B	-1.4259
363	K	B	-2.0324

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Automated mutations analysis - charged mutations

In the automated mutations mode, the server selects aggregation prone resides and each selected residue is mutated to glutamic acid, lysine, aspartic acid and arginine. The table below shows 2 best scored mutants for each mutated residue. Protein variants are ordered according to the mutation effect they had on protein stability (energetic effect) together with the difference in the average per-residue aggregation score between the wild type and the mutant (in the table green values indicate a positive change, grey are neutral, and orange/red mean destabilizing or more aggregation prone mutants).
Summary for all the mutants can be found in this CSV file .

Mutant	Energetic effect	Score comparison
LR126B	-0.8452	-0.012	View	CSV	PDB
IK76A	-0.6153	-0.0101	View	CSV	PDB
LR265A	-0.331	-0.0128	View	CSV	PDB
IR76A	-0.6253	-0.0094	View	CSV	PDB
LK126B	-0.2293	-0.0112	View	CSV	PDB
LK116A	-0.0888	-0.0109	View	CSV	PDB
LR116A	0.0278	-0.0113	View	CSV	PDB
LR370A	0.0308	-0.011	View	CSV	PDB
LK265A	0.1402	-0.0106	View	CSV	PDB
LK370A	0.1616	-0.0113	View	CSV	PDB
LR11B	0.2251	-0.0114	View	CSV	PDB
LE11B	0.3603	-0.0113	View	CSV	PDB

Project name: d3cfe2efc86c51d

Status: done

Started: 2026-05-20 07:37:39

Automated mutations analysis - charged mutations

Mutant

Energetic effect

Score comparison